Published in September 1987
Physician and Surgeon specializing in Dentistry. Periodontist.
Piazza Repubblica 4, 09129 Cagliari, Italy.
tel ++39 070 498159, fax ++39 070 400164
web site

Key words:
blood coagulation, fibrin, thrombin, tissucol
1. Tissucol is mixed with aprotinina at 87°C. Tissucol contains Fibrogen and factor XIII.
Aprotinina, by inhibiting local fibrinolysis, delays its reabsorption.
2. Lyophilized bovine thrombin is diluted with calcium chloride at 87° C. If thrombin is used at a concentration of 4 I.U./ml, solidification time is approximately one minute; at a concentration of 500 I.U./ml, solidification is all but immediate with an extremely powerful haemostatic effect. In either case, if a longer solidification time is desired it is sufficient to add 1 g of an antibiotic per ml of glue.
3. The solutions prepared are mixed in the Tissutherm device which keeps the temperature at 87°C and allows mixing without the formation of foam.
4. The solutions, Tissucol+aprotinina and thrombin+calcium chloride, are aspired into two separate syringes.
5-7. The two syringes are placed in a Duplojet syringe holder, thus keeping the two solutions separate until they come out of the needle.
8. Conversion of Fibrogen into fibrin: the factor XIII transforms the soluble fibrin into insoluble fibrin, thus stabilizing it (in practice the final stage of blood coagulation takes place).
9. Blood coagulation up to the formation of thrombin.


  1. GOTTLOW, J., NYMAN, S., LINDHE, J., KARRING, T. & WENNSTROM, J.; "New attachment formation in the Human periodontium by guided tissue regeneration. Case reports", J. Clin. Periodontol, 6: 604-615, 1986
  2. KARRING, T., NYMAN, S. & LINDHE, J.; "Healing following implantation of periodontitis affected roots into bone tissue", J, Clin. Perio- dontol., 7: 96-105, 1980
  3. MAGNUSSON, I., NYMAN, S., KARRING, T. A ELGEBERG, J.; "Connective tissue attachment formation following exclusion of gingival connective tissue and epithelium during healing", J. Period. Res., 20: 201-208, 1985
  4. NYMAN, S., GOTTLOW, J., KARRING, T. & LINDHE, J.; "The regenerative potential of the periodontal ligament. An experimental study in the monkey", J. Clin. Periodontol., 9: 257-265, 1982
  5. NYMAN, S.,KARRING, T., LINDHE, J., & PLANTEN, S.; "Healing following implantation of periodontitis affected roots into gingival connective tissue", J. Clin. Periodontol, 7: 394-400, 1980
  6. PETTI, G.; "L'impiego di membrana amniotica a protezione della zona donatrice di un innesto libero", Il Dentista Moderno, (IV) 5: 895-897, 1986
  7. PETTI, G.; "Le metodiche attuali per il trattamento chirurgico ricostruttivo dei difetti ossei", Il Dentista Moderno, (IV) 5: 817-832, 1986
  8. PETTI, G.; "L'impiego della membrana amniotica in chirurgia ossea", Il Dentista Moderno, (IV) 9: 1677-1682, 1986
  9. PETTI, G.; "Considerazioni sull'uso della membrana amniotica", Il Dentista Moderno, (V) 1: 97- 102, 1987
  10. PETTI, G.; "Lembo trapezoidale mucoperiosteo e innesto libero di gengiva per il trattamento di una fistola oro antrale cronica", Il Dentista Moderno, (III) 5: 865-870, 1985