Published in November 1989
Physician and Surgeon specializing in Dentistry. Periodontist.
Piazza Repubblica 4, 09129 Cagliari, Italy.
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Fig. 29 The specific dye shows up a thin layer of amorphous material at the cement-new gingival connective tissue interface. At some points it appears to be dissociated in fascicles of fibres (picro-sirius-hematoxylin dye). Fig. 30 Polarized light affords better definition, starting from the root and proceeding outwards the layer of cement with the characteristic arrangement of fibres in fascicles parallel and orthogonal to the root surface, the intermediate layer of amorphous tissue and finally the arrangement of woven fibres of the subepithelial connective tissue (picro-sirius-hematoxylin).
Fig. 31 The apical region: at the centre of the photograph we see the step that marks the deepest point reached with radicular curettage; the periodontal connective tissue appears clearly detached from the dentine and there are no signs of the formation of new cement. On the lower right we can see the cavity left by the bone reproducer (Interpore 200) following decalcification treatment (Azan-Mallory).

Fig. 32 The stronger enlargement shows the step where the apical cement stops and the lack of amorphous material between the cement and the periodontal connective tissue. Signs of the presence of the bone inducer show up as white (picro-sirius-hematoxylin).

Fig. 33 The previous preparation observed under polarized light confirms the lack of newly-formed cement between the periodontal connective tissue and the cement picro-sirius-hematoxylin).
Fig. 34 Histological section of the control tooth in which the bone defect was induced but left untreated: we find no short attachment, new cement, new bone apposition or organization of connective fibres at the root.
The histological pictures presented show the premises for a return to integrity of periradicular periodontal tissue and that the biological process leading to new attachment developed more quickly at the coronal rather than apical level. This can be explained both by the presence of the amniotic membrane, which in all probability delayed apical migration of the epithelium, thus favouring the formation of a short attachment, and supplied the trophic material for the deep connective tissue, and because at the apex tissue and vascular regeneration was probably delayed by the bone inducer-reproducer.
It is beyond doubt that with this technique it is possible to create the conditions for the formation of a short epithelial attachment, the formation of new cement, a new periodontal ligament and new bone formation around and inside the canaliculi of Interpore 200, thus obtaining the new attachment.


  1. BOWERS, G.M.: "Histological evaluation on new attachment in humans", Communication in Periodontal Symposium 30th Anniversary oS Pennsylvania University, giugno 1985
  2. GOTTLOW, J.; NYMAN, S.; LINDHE, J.; KARRING, T.; WENNSTROM, J.: "New attachment formation in the human periodontium by guided tissue regeneration. Case reports", J. of CL Period., 6: 604-615, 1986
  3. Karring, T.; NYMAN, S.; LINDHE, J.: "Healing following implantation of periodontitis affected roots into bone tissue", J, of CL Period., 7: 96, 105, 1980
  4. ISIDOR, F.; KARRING, T.; NYMAN, S.; LINDHE, J.: "The significance of coronal growth of periodontal legament tissue for new attachment formation", J. of Cl. Period"2: 145-150, 1986
  5. ELLEGAARD, B.: "Bone grafts in Periodontal Attachment procedures", J. of CL Period., 5 (extra issue), 1976
  6. KENNEY, E.B.; LEKOVIC, V.; HAN, T.; CARRANZA, J.R.; DIMITRISEVIC, B.: "The use of a Porous Hydroxylapatite Implant in Periodontal Defects. Clinical results after six months", J, of CL Period., 2: 56-82, 1985
  7. LINDHE, J.; WESTFELT, E.; NYMAN, S,; SOCRANSKJ, S,; HEIJL, L.; BRATTHAL, G,: "Healing following surgical/non surgical treatment of periodontal disease", J. of CL Period., 9, 1982
  8. MAGNUSSON, J.; NYMAN, S.; KARRING, T.; ELGEBERG, J.: "Connective tissue attachment formation following exclusion of gingival connective tissue and epithelium during healing", J. of Periodontal Research, 20: 201-208, 1985
  9. NYMAN, S.; "New attachment position based on the principle of guided tissue regeneration", Communication in 3rd SIdP International Congress, Milano, 4-7 giugno 1986
  10. NYMAN, S.; KARRING, T.,' LINDHE, J.,' PLANTEN, S.; "Healing following implantation of periodontitis affected roots into gingival .connective tissue", J. of Cl. Period., 7: 394-400, 1980
  11. NYMAN, S.: "Guided tissue regeneration in new attachment procedures", Communication in Periodontal Symposium 30th Anniversary of Pennsylvania University, giugno 1985
  12. PETTI, G,: "Le metodiche attuali pei il trattamento chirurgico ricostruttivo dei difetti ossei", Il Dentista Moderno, 5: 817-832, 1986
  13. PETTI, G.: "L'impiego della membrana amniotica a protezione della zona donatrice di un innesto libero", II Dentista Moderno, 5, 895-897, 1986
  14. PETTI, G.: "L'impiego della membrana amniotica in chirurgia ossea", Il Dentista Moderno, 9: 45-50, 1986
  15. PETTI, G.: "L'impianto di idrossiapatite microporosa con conservazione della papilla a protezione della zona ricevente", Il Dentista Moderno, 4: 667-677, 1986
  16. PETTI, G.: "Considerazioni sull'uso della membrana amniotica", Il Dentista Mocterno, 1: 97-102, 1987
  17. PETTI, G., "New attachment. La rigenerazione parodontale guidata con l'uso della membrana amniotica e della colla di fibrina: evoluzione di un pensiero", Piccolo Atlante di Parodontologia, cap. VIII: 57-62, Edizioni Ellebi s.r.l., 1987
  18. PETTI, G.: "Una nuova tecnica per isolare lo spazio parodontale in chirurgia ossea",Il Dentista Moderno, 3: 425-434, 1988
  19. PETTI, G.: "L'impiego della colla di fibrina in odontostomatologia", Il Dentista Moderno, 9: 1889-1908, 1987